On Thin Ice: Cryopreservation of Coral Sperm for Genetic Banking and Reef Restoration

This is where we talk about cryobiology.

According to the Society for Cryobiology, cryobiology constitutes “the study of any biological material or system (e.g., proteins, cells, tissues, and organs, insects, seeds or plant embryos) subjected to any temperature below their normal range.” This can include

It allows for long-term storage of biological material

Applications for conservation.

Challenges with cryopreservation

It is important to prevent formation of both extracellular and intracellular ice to avoid damaging cell components and membranes.

The goal is vitrification - transitioning from liquid to stable, crystal-free ice, or glass.

Permeating cryoprotective agents (CPAs) reduce water without excess salt ions inside or outside the cell.

When biological systems are pushed outside of their normal range, things usually don’t go too well.

Applications for conservation.

Next steps?